Separation Science, in collaboration with GE Healthcare,
offers an educational microsite focusing on the use of
size exclusion chromatography (SEC) for protein analysis.

SEC is a powerful chromatography technique for obtaining reliable information about the size of biomolecules under native conditions. As such, it is widely used in several different analytical applications from basic research to quality control of biotherapeutics. This educational microsite presents fundamentals about analytical SEC, tips to ensure successful analysis, available products and applications showcasing the role of SEC in biomolecule analysis. So what's included in the microsite...

Protein analysis white paper
Presents fundamentals about analytical SEC and describes how
new-generation agarose-based SEC columns are used in analysis of biomolecules. Topics covered include:

• Introduction to protein analysis with SEC
• How to select an SEC column for protein analysis
• Resin properties
• Column-related factors
• Running conditions
• Liquid chromatography system configuration
• and much more...

Tips for improving analytical SEC
The possibility to separate molecules by size under native conditions is powerful in protein analysis. With over thirty years in protein science, Åke Danielsson, Research Director at GE Healthcare, recently held a webinar on protein characterization using size exclusion chromatography (SEC). This article shares three important takeaways from that webinar.

Analysis of membrane conformational changes
This application uses the Superdex™ 200 Increase 5/150 GL column for size in the analysis of conformational changes of the CorA magnesium channel in response to Mg²⁺ binding. SEC allowed for rapid analysis of protein complex formation under non-denaturing conditions.