Video Tutorials

Finding the Right Particle Size and Pore Size
In this video we'll discuss in more detail how to avoid strange peak shapes in GPC/SEC and on what criteria correct column selection should be based. If the question 'I have completely new samples and need to identify the best columns to analyze them' sounds familiar then this tutorial will help you out.

Unexpected Peak Shapes
Do you find weird peak shapes in your chromatograms? Not sure if they are real or artifacts? In this 25-minute tutorial you'll learn about peak shapes and how to verify if a GPC/SEC chromatogram is realistic or not.

Pressure Problems
In this tutorial you'll discover what strategies can be implemented to overcome pressure problems in your liquid chromatography system.

What Should You Know Before Injecting Your First Sample
In this tutorial you are introduced to typical components in GPC/SEC and will discover what you need to know about your GPC/SEC system? You will also learn what plate count means and how can you determine it.

Applications

Separation of Statistic MMA-MAA Copolymers using Gradient SEC
Statistic copolymers of methyl methacrylate (MMA) and methacrylic acid (MAA) are widely used in pharmaceutical applications. Besides the molar mass distribution, the chemical composition and the amount of comonomers in the copolymer is of importance. SEC-gradients is the method of choice to characterize these samples.

EP/USP GPC/SEC Molar Mass Determination for Low Molecular Weight Heparin
In this application, GPC/SEC is used to measure the molecular weight (molar mass) and the molecular weight fractions above/below a molecular weight
limit using a procedure that complies with both EP and USP standards.

GPC/SEC-Light Scattering for Starch Analysis
Starches are polysaccharides produced by all green plants to store energy. The study of native starches from different natural sources shows that GPC/SEC-MALLS can be used efficiently to perform molecular characterization and degradation monitoring.

About the Video Presenter

Dr Daniela Held studied chemistry at the University of Mainz. Her PhD work was on the characterization of star-branched polymers using GPC/SEC and hyphenated techniques. She joined PSS in 2000 and is responsible for customer training and support.